Sulfo-NHS-LC-Biotin, Sulfosuccinimidyl 6-(biotinamido) Hexanoate, is a long chain, water soluble biotinylation reagent that is reactive toward primary amine groups.Similar to Sulfo-NHS-Biotin, Sulfo-NHS-LC-Biotin is designed to target cell surface proteins. The sodium sulfonate groups lend water solubility to Sulfo-NHS-LC-Biotin and prevent it from crossing the lipid bi-layer of the cell membrane; whereas NHS-LC-Biotin can biotinylate intracellular proteins.The resulting biotinylated protein can then be detected when used in conjunction with streptavidin and Immobilized Streptavidin.The "long chain" Sulfo-NHS-LC-Biotin and Sulfo-NHS-Biotin both have the same chemical functionality.However, Sulfo-NHS-LC-Biotin will provide a longer spacer arm that is designed to overcome any steric hindrances that may be present between the labeled protein(s) and streptavidin (ProteoChem product # e7105-5mg).Often, the best results are determined by empirically testing each reagent for your specific application.

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Molecular Weight: 556.59Spacer Length: 22.4 ÅCAS Number: 127062-22-0Alternative Names: Sulfosuccinimidyl-6-(biotinamido)hexanoate; Biotin-X-NHS, Water-Soluble; Biotinamidohexanoic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt; Sulfo NHS LC Biotin; sulfosuccinimidyl biotinamido hexanoate; sulfo succinimidyl biotinamido hexanoate; Biotinamidocaproate N-hydroxysulfosuccinimidyl ester; sulfosuccinimidyl biotinamidyl hexanoate; long chain Sulfo NHS BiotinReference:Senchenkova, E. Y., Komoto, S., Russell, J., Almeida-Paula, L. D., Yan, L. S., Zhang, S., & Granger, D. N. (2013). Interleukin-6 Mediates the Platelet Abnormalities and Thrombogenesis Associated with Experimental Colitis. The American journal of pathology.Special bulk pricing is available on bulk quantities of Sulfo-NHS-LC-Biotin for your large volume manufacturing application.