Immobilized Pepsin resin (Pepsin Agarose) is commonly used to create F(ab)2 fragments from antibodies.By immobilizing pepsin autolysis and protease contamination is essentially eliminated.Immobilized Pepsin gel hydrolyzes protein non-specifically at pH 1.0 to 3.0, with an optimum activity at pH 1.0. ProteoChem"s highly active pepsin is covalently bound to crosslinked 6% beaded agarose resin and its specific activity is > 6,100 units/ml resin (one unit is defined as releasing 0.001 A280 as TCA hydrolyzed/min. at 37°C).ProteoChem"s Immobilized Pepsin is packaged as a slurry in buffer at pH 4.5 containing 50% glycerol, 100 mM Sodium Acetate, 0.05% NaN3.Pepsin protease cleaves non-specifically with some preference for tyrosines and phenylalanine (aromatic residues), although it generally does not cleave at alanine, glycine or valine residues. Recommended conditions are pH 1.0-4.5, 20°C to 37°C.
Click here for: Immobilized Pepsin Product Information Sheet & General Protocol
Special bulk pricing is available on bulk quantities of Immobilized Pepsin for your large volume manufacturing application.
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ProteoChem公司将产品的高质量作为最高标准,每一个产品都要通过严格的质量监测体系检测,确保了稳定可靠的产品。ProteoChem公司的目标是提供卓越的产品和高效的技术支持,同时还努力开发更先进的检测系统,保证客户能得到代表最高水平的服务。从研究与临床需要出发,设计开发产品,真正满足实际要求,产品已被生命科学研究群体所广泛认同;有完善的专业文献资料积累,所有产品都提供详细的技术资料与重要的相关文献;产品提供多种规格产品参数:ProteoChem produces ultrapure matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) matrix products. ProteoChem's UltraPure MALDI-MS matrices are purified by a propriety method designed to yield MALDI mass spectra with increased sensitivity, better resolution, and high signal to noise ratios. ProteoChem's MALDI matrices are specialized and intended for analysis of proteins and peptides.Please follow the links below to see our UltraPure MALDI matrix pricing and product information: CHCADHBSinapinic AcidHABATHAPDHAPMBTMALDI Matrix Sampler Pack