PDPH crosslinker (3-[2-Pyridyldithio]propionyl hydrazide) is a cleavable, carbohydrate reactive, heterobifunctional protein crosslinking reagent.The PDPH crosslinker"s hydrazide group selectively reacts with carbohydrates oxidized by sodium meta-periodate (or carboxyls when used with EDC); while the pyridinyldisulfide moity is reactive toward reduced thiols (sulfhydryls; -SH).Thus, PDPH protein crosslinker is an excellent choice for crosslinking glycoproteins, glycans, and other polysaccharides.Additionally, the pyridyldisulfide group of the PDPH crosslinking reagent contains a cleavable disulfide (-S-S-) bond which can be reduced with TCEP-HCl or DTT to expose a thiol moity that can be further reacted with, for example, a maleimide containing crosslinker.Thus, PDPH may also be used as a thiolation reagent to add sulfhydryl functional groups to carbohydrate containing molecules.PDPH is soluble at 14.2 mg/mL in pH 5.5 0.1 M Sodium Acetate.
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Molecular Weight: 229.32Spacer Length: 9.2 ÅAlternative Names: SPDP Hydrazide; PDPH Cross-linker; PDPH Cross Linker; S-(2-thiopyridyl)-3-mercaptopropionic acid hydrazide; TPMPH; 3-(2-pyridyldithio)propionyl hydrazideSpecial bulk pricing is available on bulk quantities of PDPH crosslinker for your large volume manufacturing application.
